5(6)-Carboxyfluorescein N-succinimidyl ester | High Purity Fluorescent Reagent
5(6)-Carboxyfluorescein N-succinimidyl ester CAS 117548-22-8
- Surfactant Appearance: Powder
- Proportion: Minimum 99.0%
- Inventory: In stock
- Sample: In stock
- Zhishang Chemical: Supplier and manufacturer of 5(6)-Carboxyfluorescein N-succinimidyl ester CAS 117548-22-8
Basic Info of 5(6)-Carboxyfluorescein N-succinimidyl ester
| Dibutyl phthalate Chemical Properties |
| Melting point | -35 °C (lit.) |
| Boiling point | 340 °C (lit.) |
| density | 1.043 g/mL at 25 °C (lit.) |
| vapor density | 9.6 (vs air) |
| vapor pressure | 1 mm Hg ( 147 °C) |
| refractive index | n20/D 1.492(lit.) |
| Fp | 340 °F |
| storage temp. | 2-8°C |
| solubility | Very soluble in alcohol, ether, acetone, benzene |
| form | Liquid |
What is 5(6)-Carboxyfluorescein N-succinimidyl ester？
5(6)-Carboxyfluorescein N-succinimidyl ester (shortened as 5( 6 )- FAM SE), CAS number 117548-22-8, is an amine-reactive environment-friendly fluorescent color.
Its core structure consists of a traditional fluorescein chromophore and an N-hydroxysuccinimide active ester, with a molecular weight of about 473.39. 5(6)-Carboxyfluorescein N-succinimidyl ester normally exists as a blend of isomers replaced at the 5- and 6-positions with carboxyl groups.
The vital attribute of 5(6)-Carboxyfluorescein N-succinimidyl ester lies in its very details succinimidyl ester group, which responds with primary amines in healthy proteins, antibodies, or synthetic peptides under neutral to weakly alkaline problems to create stable amide bonds.
In comparison, traditional FITC labeling reagents form thiourea bonds via isothiocyanate responses, whereas the amide bond item of 5(6)-Carboxyfluorescein N-succinimidyl ester offers superior chemical security, is much less vulnerable to hydrolysis, and is consequently better suited for long-lasting tracking experiments.
In terms of photophysical residential properties, 5(6)-Carboxyfluorescein N-succinimidyl ester has an optimum excitation wavelength (Ex lover) of around 492 nm and an optimum discharge wavelength (Em) of about 517 nm, dropping within the standard green fluorescent channel and compatible with standard FITC filter sets used in research laboratories.
It has a high fluorescence quantum return, stable fluorescence intensity under pH > 6 problems, and level of sensitivity enough for various applications ranging from flow cytometry to high-resolution microscopy.
Product Packaging
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Core Applications and Supply Notes
In the field of life sciences, 5(6)-Carboxyfluorescein N-succinimidyl ester largely acts as a signaling probe.
( 1) General healthy protein labeling: typically utilized to prepare fluorescent antibodies or enzyme-labeled additional antibodies for ELISA, Western blot, or immunofluorescence assays.
( 2) Cell expansion tracking (CFSE): although the traditional cell spreading dye CFSE is its diacetate form, it shares the same core framework as this substance. Upon entering cells, it requires activation by esterases and covalently binds to intracellular healthy proteins; this application is frequently made use of in immunology to spot lymphocyte expansion.
( 3) Small molecule mapping: can be used to synthesize fluorescently classified substratums or medications for assessing medication circulation or metabolic paths in vivo.
When offering 5(6)-Carboxyfluorescein N-succinimidyl ester, we stress that its succinimidyl ester is highly susceptible to hydrolysis and will end up being inactive upon contact with water or moisture. Therefore, it should be kept strictly at -20 ° C in a dry, sealed atmosphere. The vial should be offered space temperature level prior to opening up, and anhydrous dimethyl sulfoxide or dimethylformamide ought to be utilized when preparing remedies.
FAQ
It is mainly used for biomolecular labeling, cell staining, immunoassay and related biochemical experiments.
Our product has high purity and stable properties, meeting the standards for laboratory and industrial research use.
Yes, small quantity samples are available for your quality and performance testing.
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5(6)-Carboxyfluorescein N-succinimidyl ester Supplier and Manufacturer
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