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Bromothymol Blue

Zhishang Bio Bromothymol Blue CAS 76-59-5

Payment: L/C,T/T,D/P,Paypal,Money Gram,Western Union

Incoterm: FOB,CFR,CIF,EXW,FCA,CPT,CIP

Transporta: Ocean, Land,Air, DHL,TNT

Port: Shanghai Port, Qingdao Port

Description

1.Bromothymol Blue is also called Bromothymol Blue and Bromothymol Blue. It belongs to the class of sulfophthalein dyes. Its molecular composition is C 27H 28 Br 2O 5 S with a molecular mass of 624.38. The CAS number for this dye is 76-59-5.
Bromothymol blue is centered on a benzothiadiazole, with two bromothymol group attached at position 3.
The conjugated structure of Bromothymol Blue allows for it to undergo conformational changes when the pH changes.

2. Physical characteristics and morphology

Bromothymol blue is a light red to purple powder that is soluble in ethanol and acetone. It is also soluble in dilute alkaline solution, but is almost insoluble with petroleum ether. As a pH indicator, the range of color changes is near neutral: pH 6.0 yellow to pH 7.6 blue.
It appears green in a neutral solution, with a pH around 7.0. This is the result from a mix of yellow and blue.

3. The preparation method and the industry positioning

Bromothymol is prepared by bromination, which involves adding bromine atoms to the thymol molecule in order to change its optical properties and color range. It is used as a pH indicator in industrial production and laboratories because its range of color changes lies in the neutral pH zone.
Bromothymol blue has an advantage over phenolphthalein, which is a color indicator for the alkaline zone.

Product Images & Packaging

Bromothymol Blue
Bromothymol Blue
Bromothymol Blue

Bromothymol Blue Parameters

Bromothymol Blue Chemical Properties
Melting point 200-202 °C (lit.)
bulk density450kg/m3
density 1.4668 (estimate)
Fp 38 °C
storage temp. Store at +5°C to +30°C.
solubility Solubility Sparingly soluble in water, benzene; soluble in ethanol, ether; insoluble in petroleum ether
pka7.0, 7.1(at 25℃)
form Solid
Boiling point 614.3±55.0 °C(Predicted)
color Pink-brown to purple
OdorCharacteristic odour
PHpH : 6.0~7.6
PH Range6(yellow)-7.6(blue)

The application of Bromothymol Blue

Bromothymol blue: its main applications

Bromothymol blue, as an acid-base indicator of neutral range, is used in many fields, including biological experiments, environmental monitoring and industrial production, due to its good water solubility and sensitive color reaction.

Field of biological experimentation

Bromothymol blue is used to detect carbon dioxide production in biological research. Bromothymol Blue’s gradual change from blue to green to yellow to acidic in photosynthesis and respiration can be intuitively interpreted as a CO 2 concentration change.
The CO2 released from the respiration process of living seeds is dissolved in water and forms carbonic acid. This increases the acidity. Bromothymol Blue turns yellow when it changes color from blue. This indicates the viability.

Bromothymol Blue can be used in aquatic biology experiments as a respiratory agent for aquatic organisms. For a short time, the organisms are unaffected by this indicator. The accumulation of CO2 produced by metabolic processes can be measured through changes in color of water.


Environmental monitoring and Clinical Diagnosis Field

Bromothymol Blue can be used to detect pH in fish tanks and swimming pools. The color change range (pH 6.6-7.6) is the same as the range of daily fluctuations in neutral water bodies. It can be used to quickly determine if the water is suitable for biological life through its color. This indicator can be used in food freshness tests to monitor the acidic metabolic products produced by microbial replication during food storage.

Bromothymol blue is used in the clinical field to detect premature membrane rupture by measuring pH changes in vaginal fluid (normally acidic; amniotic liquid weakly alkaline).
This helps in diagnosing. It is also added to culture media in microbial tests to monitor pH variations caused by metabolic acid formation during bacterial growth.

Industry and Laboratory Analysis Field

Bromothymol blue is a common indicator in chemical analysis. It is especially useful for weak acid or weak base titration. Its color-change point (pH around 7.0) is near the titration’s endpoint.
This allows accurate endpoint determination.

It is used to determine acidity in gases in industrial applications by detecting color changes. Bromothymol Blue can be mixed with the matrix or coating of a sol gel to create pH-sensitive functional materials. These are used for anti-corrosion monitoring or intelligent packaging. It is also used in optical materials to create pH-sensitive components for fuel cell.


Bromothymol blue is used in the textile, printing, and dyeing industries as a dye to color specific fabrics. It can also be used for staining biochemical sections for observing nucleus or cell wall structures under a microscopy.

Bromothymol blue is a classic chemical indicator that has remained relevant in modern science. It can be used to observe photosynthesis during high school biology lessons and as a sentinel in monitoring water quality for industrial production.

Due to their intuitive, sensitive and reliable properties, these compounds, synthesized more than a century ago, continue to play a vital role in a wide range of settings, including laboratories, factories, classrooms, and clinical settings.

Bromothymol Blue 's Procurement Guide

Procurement Guidelines
1. Sample Policy
We can provide a 2-3g free trial package (shipping fee required)

2. Batch procurement
Packaging: 25kg/bag; 220kg/barrel; Special packaging according to your needs
MOQ:1kg

3. Customized services
Concentration customization (90% -99%)
Paeonol’s outsourcing services

Paeonol

FAQ

Laboratory: Acid base titration, precise indication of neutral pH (6.0-7.6)
Biomedical: Cell staining, respiratory indicators (measuring CO ₂), detection of premature rupture of membranes
Environment/Aquatic Products: pH Monitoring of Pool Water, Fish Tank Water, and Cooling Water
Food/Industry: Milk adulteration detection, pH monitoring during fermentation process

Bromothymol Blue: pH 6.0-7.6 (yellow → blue), neutral zone specific
Thymol Blue: pH 1.2-2.8/8.0-9.6 (dual color)
Bromophenol blue: pH 3.0-4.6 (yellow → purple), electrophoretic tracer

Sealed, * * dry, dark, stored at room temperature (5-30 ℃) * *
Stable for 2 years; Avoid strong oxidants, strong alkalis, and moisture
Ready to use solution or short-term storage at 4 ℃

Related References

Manufacturer of Bromothymol Blue

Company Info

Zhishang Bio company in China that exports chemicals, Cosmetic raw materials, and Intermediates for over 70 countries now.
 
And a range of 50,000 products with the best quality and competitive price to meet your different purchasing needs.
 
The company integrates research and development, production, and trade, with a modern production base, advanced testing equipment, and an experienced technical team to ensure that products reach industry-leading levels in purity, stability, and environmental friendliness.

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