
PRODUCT
Description
Bromothymol blue is centered on a benzothiadiazole, with two bromothymol group attached at position 3. The conjugated structure of Bromothymol Blue allows for it to undergo conformational changes when the pH changes.
2. Physical characteristics and morphology
Bromothymol blue is a light red to purple powder that is soluble in ethanol and acetone. It is also soluble in dilute alkaline solution, but is almost insoluble with petroleum ether. As a pH indicator, the range of color changes is near neutral: pH 6.0 yellow to pH 7.6 blue. It appears green in a neutral solution, with a pH around 7.0. This is the result from a mix of yellow and blue.
3. The preparation method and the industry positioning
Bromothymol is prepared by bromination, which involves adding bromine atoms to the thymol molecule in order to change its optical properties and color range. It is used as a pH indicator in industrial production and laboratories because its range of color changes lies in the neutral pH zone. Bromothymol blue has an advantage over phenolphthalein, which is a color indicator for the alkaline zone.
Product Images & Packaging



Bromothymol Blue Parameters
| Bromothymol Blue Chemical Properties |
| Melting point | 200-202 °C (lit.) |
| bulk density | 450kg/m3 |
| density | 1.4668 (estimate) |
| Fp | 38 °C |
| storage temp. | Store at +5°C to +30°C. |
| solubility | Solubility Sparingly soluble in water, benzene; soluble in ethanol, ether; insoluble in petroleum ether |
| pka | 7.0, 7.1(at 25℃) |
| form | Solid |
| Boiling point | 614.3±55.0 °C(Predicted) |
| color | Pink-brown to purple |
| Odor | Characteristic odour |
| PH | pH : 6.0~7.6 |
| PH Range | 6(yellow)-7.6(blue) |
The application of Bromothymol Blue
Bromothymol blue: its main applications
Bromothymol blue, as an acid-base indicator of neutral range, is used in many fields, including biological experiments, environmental monitoring and industrial production, due to its good water solubility and sensitive color reaction.
Field of biological experimentation
Bromothymol blue is used to detect carbon dioxide production in biological research. Bromothymol Blue’s gradual change from blue to green to yellow to acidic in photosynthesis and respiration can be intuitively interpreted as a CO 2 concentration change. The CO2 released from the respiration process of living seeds is dissolved in water and forms carbonic acid. This increases the acidity. Bromothymol Blue turns yellow when it changes color from blue. This indicates the viability.
Bromothymol Blue can be used in aquatic biology experiments as a respiratory agent for aquatic organisms. For a short time, the organisms are unaffected by this indicator. The accumulation of CO2 produced by metabolic processes can be measured through changes in color of water.
Environmental monitoring and Clinical Diagnosis Field
Bromothymol Blue can be used to detect pH in fish tanks and swimming pools. The color change range (pH 6.6-7.6) is the same as the range of daily fluctuations in neutral water bodies. It can be used to quickly determine if the water is suitable for biological life through its color. This indicator can be used in food freshness tests to monitor the acidic metabolic products produced by microbial replication during food storage.
Bromothymol blue is used in the clinical field to detect premature membrane rupture by measuring pH changes in vaginal fluid (normally acidic; amniotic liquid weakly alkaline). This helps in diagnosing. It is also added to culture media in microbial tests to monitor pH variations caused by metabolic acid formation during bacterial growth.
Industry and Laboratory Analysis Field
Bromothymol blue is a common indicator in chemical analysis. It is especially useful for weak acid or weak base titration. Its color-change point (pH around 7.0) is near the titration’s endpoint. This allows accurate endpoint determination.
It is used to determine acidity in gases in industrial applications by detecting color changes. Bromothymol Blue can be mixed with the matrix or coating of a sol gel to create pH-sensitive functional materials. These are used for anti-corrosion monitoring or intelligent packaging. It is also used in optical materials to create pH-sensitive components for fuel cell.
Bromothymol blue is used in the textile, printing, and dyeing industries as a dye to color specific fabrics. It can also be used for staining biochemical sections for observing nucleus or cell wall structures under a microscopy.
Bromothymol blue is a classic chemical indicator that has remained relevant in modern science. It can be used to observe photosynthesis during high school biology lessons and as a sentinel in monitoring water quality for industrial production.
Due to their intuitive, sensitive and reliable properties, these compounds, synthesized more than a century ago, continue to play a vital role in a wide range of settings, including laboratories, factories, classrooms, and clinical settings.
Bromothymol Blue 's Procurement Guide
Procurement Guidelines
1. Sample Policy
We can provide a 2-3g free trial package (shipping fee required)
2. Batch procurement
Packaging: 25kg/bag; 220kg/barrel; Special packaging according to your needs
MOQ:1kg
3. Customized services
Concentration customization (90% -99%)
Paeonol’s outsourcing services

FAQ
Laboratory: Acid base titration, precise indication of neutral pH (6.0-7.6)
Biomedical: Cell staining, respiratory indicators (measuring CO ₂), detection of premature rupture of membranes
Environment/Aquatic Products: pH Monitoring of Pool Water, Fish Tank Water, and Cooling Water
Food/Industry: Milk adulteration detection, pH monitoring during fermentation process
Bromothymol Blue: pH 6.0-7.6 (yellow → blue), neutral zone specific
Thymol Blue: pH 1.2-2.8/8.0-9.6 (dual color)
Bromophenol blue: pH 3.0-4.6 (yellow → purple), electrophoretic tracer
Sealed, * * dry, dark, stored at room temperature (5-30 ℃) * *
Stable for 2 years; Avoid strong oxidants, strong alkalis, and moisture
Ready to use solution or short-term storage at 4 ℃
Related References
Manufacturer of Bromothymol Blue
Zhishang Bio is based in Shandong, serves the whole country, faces the world, and is determined to become an excellent supplier and service provider in the chemical industry.
As a top manufacturer of cosmetic raw materials, disinfectants, flavors and fragrances in China, we are committed to producing high-quality products and have won high praise from customers all over the world.
In the next 10 years or even longer, Zhishang Bio will always adhere to the tenet of quality first and reputation first, and devote itself to providing customers with thoughtful and perfect services.
As a professional Bromothymol Blue manufacturer, Zhi shang Bio has served more than 6,000 customers around the world. Because of our high-quality products and professional services, we have won high praise from most of our customers.
Zhishang Bio is a professional enterprise engaged in the research and development, production, and sales of chemical raw materials. It has a strong supply chain system and strict quality control standards to ensure the provision of high-quality products with high purity and stability to customers.
Zhishang Bio’s core strength is its independent research and design capabilities. It has advanced production equipment, testing laboratories and can customize chemical raw materials to meet the market demands. This includes pharmaceuticals, electronic, coatings and agricultural applications.
Zhishang Bio’s supply chain management is based on long-term relationships with domestic and international suppliers of high quality to guarantee a stable supply, optimize logistic systems, and achieve efficient distribution. This helps reduce the cost of customer purchases.
We adhere to international environmental standards and we also adhere to the concept that environmental protection and safety production are important. All production processes must comply with these standards in order to guarantee that our products will be green and sustainable.
With a professional technical team and comprehensive after-sales service, Zhishang Bio can provide customers with precise technical support, solve application problems, and help customers enhance product competitiveness.
We are always customer-oriented and committed to becoming a trusted chemical raw material partner.
Zhishang Biography is dedicated to using clients extensive and expert service, including chemical raw material. The customers will certainly receive hassle-free, efficient and one-stop options.
1. Custom-made Supply Solutions
Zhishang Bio provides tailored services for clients in the chemical basic material sector, including special bit dimension, solvent residue control, stability optimizations and more. Clients can personalize their services to satisfy details requirements in industries such as drugs, electronic devices, and finishings.
2. Strict quality assurance
Zhishang Bio utilizes advanced screening equipment and stringent quality examination procedures, in addition to various other files, to guarantee that every batch of products is certified with international criteria such ISO GMP, REACH, and so on. Customers are also given with full quality records and conformity documents.
The supply chain is secure
Zhishang Bio has actually established lasting calculated collaborations with worldwide makers of top notch raw materials to make certain a secure supply, maximize the storage facility and logistics system, and assistance both small-scale examinations and large orders. It adjusted to the changing requirements of clients by responding flexibly to these changes.
4. Technical support
Zhishang Bio’s technical group can offer suggestions on product applications, formula optimization and analysis of industry patterns to aid clients fix technological troubles during production and boost item efficiency.
5. Efficient after-sales service
Zhishang Bio provides complete monitoring, reacts to client requests and delivers promptly. This will certainly make certain customer complete satisfaction.
Zhishang Biography intends to help consumers expand their service by providing the very best solutions and chemical resources.
DO YOU ACCEPT SAMPLE ORDER?
We will make samples before mass production. After the samples are approved by the department and users, we will start mass production. During the production process, we will carry out 100% inspection, and then carry out random inspection before packaging.
WHAT’S YOUR MOQ?
Our MOQ is 1kg. But usually we can also accept a smaller quantity, such as 100g, if you need it, provided that the sample fee is 100% paid.
IS THERE A DISCOUNT?
Different quantity has different discount.
HOW TO CONFIRM WHETHER THE PRODUCT QUALITY MEETS OUR NEEDS BEFORE PLACING AN ORDER?
You can consult us to get free samples of some products, you only need to pay the freight or arrange express delivery to us and get the samples. You can send us the product specifications and requirements you need, and we will manufacture products according to your requirements.
DO YOU SUPPLY PRODUCT REPORT?
Yes. We’ll give you product analysis report before shipping.
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Shandong Look Chemical
1. For products ≤50kg, we recommend using express delivery, which is usually called DDU service (discounted, convenient).
2. For products ≤500kg, we generally recommend air freight, which is usually called FOB, CFR or CIF service (fast and efficient).
3. For products >500kg, we generally recommend shipping by sea, which is usually called FOB, CFR or CIF service (economical, safe).
4. For high-value products, please choose air or express to ensure the safety of product transportation.
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