PRODUCT
Coomassie brilliant blue CAS 6104-59-2
CAS:6104-59-2 | C45H46N3NaO7S2 |MW：827.99 | EINECS NO：228-060-5
Payment: L/C,T/T,D/P,Paypal,Money Gram,Western Union
Incoterm: FOB,CFR,CIF,EXW,FCA,CPT,CIP
Transporta: Ocean, Land,Air, DHL，TNT
Port: Shanghai Port, Qingdao Port
Description
Acid Blue 83 is also known as Coomassie Brilliant Blue, a synthetic dye made from triphenylmethane. The name “Coomassie Brilliant Blue”, which comes from the discoverer of the dye and the initial commercial company that produced it, is a synthetic triphenylmethane dye. Coomassie bright blue, from a chemical perspective, is a highly-conjugated organic molecule, with a triphenylmethane structure composed of three aromatic ring connected by a carbon atom in the middle, and a sulfonic group attached to it. This sulfonic group is what allows Coomassie Brilliant Blue to be water-soluble and form sodium or potassium salts. It is therefore classified as “acidic”. Coomassie Brilliant blue can both dissolve in water and bind positively charged fibers via ionic bonding in acidic dye baths. In commercial products, Coomassie brilliant blue is usually a fine powder in brownish purple or deep purple red. This ensures rapid dissolution and uniform color. Coomassie Brilliant Blue’s most notable physical properties are its unique color expression and excellent solubility. It is easily soluble in water and forms a bright, clear blue-purple solution. Coomassie Brilliant Blue is also soluble in polar organic solvants such as ethanol and methanol. However, it has poor solubility with non-polar solvants. The solution of Coomassie Brilliant Blue has a high color strength. This means that it is able to produce rich and clear colors even at very low concentrations. This is due to its high molar absorbtivity which allows it to absorb light. Coomassie Brilliant Blue also exhibits an important and well-known physicochemical property: its binding to proteins. When it binds with the hydrophobic area of the protein, the maximum absorption peak undergoes redshifting, and the absorbance of that wavelength is markedly increased. It is because of this property that it has become a widely used biochemical reagent. The Coomassie Brilliant Blue exhibits excellent chemical stability both in the solid and solution state, but it may slowly degrade over time under extreme pH conditions.
Product Images & Packaging
Product Parameters
| Acid Blue 83 Chemical Properties |
| Melting point | 174-180 °C |
| bulk density | 250kg/m3 |
| vapor density | 28.5 (vs air) |
| Fp | 14 °C |
| storage temp. | room temp |
| solubility | H2O: soluble10mg/mL |
| form | Powder/Solid |
| Colour Index | 42660 |
| color | Red Brown |
| PH | 6.2 (25°C, 10g/L in H2O) |
| Water Solubility | Soluble in water. |
| λmax | 589.2 nm in ethanol |
| BRN | 5718025 |
| Stability: | Light Sensitive |
| InChIKey | NKLPQNGYXWVELD-UHFFFAOYSA-M |
The application of Coomassie brilliant blue
Coomassie Brilliant Blue is used for a variety of purposes.
Acid Blue 83 is also known as Coomassie Brilliant Blue. It’s a “dual-star” product that can be used in industrial dyeing or life science research.
1. Key reagents for biochemistry and molecular biological research
The most popular and irreplaceable use of Coomassie Brilliant Blue in modern laboratories is based on the specific binding it has to protein molecules.
Coomassie Brilliant Blue is used in the classic application. The researchers stained the gel using the dye solution containing Coomassie Brilliant Blue as the main component after SDS polyacrylamide electrophoresis.
According to this principle, dye molecules are bound specifically to the hydrophobic regions of proteins by van der Waals interactions and hydrophobic forces. After binding, the dye-protein complex absorbance in the visible light area is greatly enhanced. The colorless bands of protein in the gel now appear blue.
This method is called “Coomassie Brilliant Blue Staining Method”. Coomassie Brilliant Blue has a lower sensitivity than silver staining but it is more convenient, faster, cheaper, offers a wider linear range and is compatible with most proteins. It is now a standard for routine protein quantification and detection in laboratories.
Bradford protein quantification was developed based on the binding principle above. Coomassie Brilliant Blue G250, which is the most commonly used dye in this method, can be used to determine protein concentrations based on similar principle. A linear relationship exists between the change in absorbance due to the dye binding to the proteins and the protein concentration in a given range. This allows for the determination of the concentration of proteins using a spectrophotometer.
Staining agents, tracers and tracer:
Coomassie Brilliant Blue can be used to enhance the contrast in biological microscopy. It can also be combined with other dyes for a more intense contrast.
It is added to the buffer in agarose gel electrophoresis as a leading marker to monitor the migration of DNA fragments. This is because the small molecules of dye run ahead of the DNA fragments.
2. Textile and Fiber Dyeing
Coomassie Brilliant Blue can be used in the same way as an acidic color, but primarily for positively charged fibers.
It can also give silk fabrics vibrant and bright blue-purple tones while maintaining their unique luster.
Coomassie brilliant can be used to dye wool yarns, fabrics and finished products. It provides a deep blue color with good fastness.
3.Color paper and leather
Coomassie brilliant can be used for a variety of colored papers such as art paper, gift wrap paper, decorative paper and more. The paper can have a unique look thanks to its brightly colored light.
Coomassie brilliant can be used to dye leather, as it can penetrate the leather’s interior and produce an uniform translucent appearance. It can be used to dye clothing leather in blue or purple, as well as furniture leather and luggage leather.
Coomassie brilliant blue 's Procurement Guide
Procurement Guidelines
1. Sample Policy
We can provide a 2-3g free trial package (shipping fee required)
2. Batch procurement
Packaging: 25kg/bag; 220kg/barrel; Special packaging according to your needs
MOQ：1kg
3. Customized services
Concentration customization (90% -99%)
Paeonol’s outsourcing services
FAQ
Yes, we have our own factory called ” ZhiShang Industry Group “
Yes, we have GMP certificate for some products, and we can provide DMF as well.
-Quality you require, eg. Assay, Purity, or single impurity
-Quantity you need
-Standard you want, such as USP, EP, JP etc.
Escrow and T/T in advance are highly recommended. We can accept Western Union, Money Gram and Paypal as well.
For sample(less than 1kg), in about 3 working days after payment; for larger orders (more than 1kg), in about 7 working days after payment.
For samples, we will send by TNT/DHL/FEDEX/UPS express, usually it takes about 3-5 day.
Related References
Manufacturer of Coomassie brilliant blue
Achievements
Advantage
Service
FAQ
Advice on Shipping
Achievements
Zhishang Bio is based in Shandong, serves the whole country, faces the world, and is determined to become an excellent supplier and service provider in the chemical industry.
As a top manufacturer of cosmetic raw materials, disinfectants, flavors and fragrances in China, we are committed to producing high-quality products and have won high praise from customers all over the world.
In the next 10 years or even longer, Zhishang Bio will always adhere to the tenet of quality first and reputation first, and devote itself to providing customers with thoughtful and perfect services.
As a professional Coomassie brilliant blue manufacturer, Zhi shang Bio has served more than 6,000 customers around the world. Because of our high-quality products and professional services, we have won high praise from most of our customers.
Advantage
Zhishang Bio is a professional enterprise engaged in the research and development, production, and sales of chemical raw materials. It has a strong supply chain system and strict quality control standards to ensure the provision of high-quality products with high purity and stability to customers.
Zhishang Bio’s core strength is its independent research and design capabilities. It has advanced production equipment, testing laboratories and can customize chemical raw materials to meet the market demands. This includes pharmaceuticals, electronic, coatings and agricultural applications.
Zhishang Bio’s supply chain management is based on long-term relationships with domestic and international suppliers of high quality to guarantee a stable supply, optimize logistic systems, and achieve efficient distribution. This helps reduce the cost of customer purchases.
We adhere to international environmental standards and we also adhere to the concept that environmental protection and safety production are important. All production processes must comply with these standards in order to guarantee that our products will be green and sustainable.
With a professional technical team and comprehensive after-sales service, Zhishang Bio can provide customers with precise technical support, solve application problems, and help customers enhance product competitiveness.
We are always customer-oriented and committed to becoming a trusted chemical raw material partner.
Service
Zhishang Biography is dedicated to using clients extensive and expert service, including chemical raw material. The customers will certainly receive hassle-free, efficient and one-stop options.
1. Custom-made Supply Solutions
Zhishang Bio provides tailored services for clients in the chemical basic material sector, including special bit dimension, solvent residue control, stability optimizations and more. Clients can personalize their services to satisfy details requirements in industries such as drugs, electronic devices, and finishings.
2. Strict quality assurance
Zhishang Bio utilizes advanced screening equipment and stringent quality examination procedures, in addition to various other files, to guarantee that every batch of products is certified with international criteria such ISO GMP, REACH, and so on. Customers are also given with full quality records and conformity documents.
The supply chain is secure
Zhishang Bio has actually established lasting calculated collaborations with worldwide makers of top notch raw materials to make certain a secure supply, maximize the storage facility and logistics system, and assistance both small-scale examinations and large orders. It adjusted to the changing requirements of clients by responding flexibly to these changes.
4. Technical support
Zhishang Bio’s technical group can offer suggestions on product applications, formula optimization and analysis of industry patterns to aid clients fix technological troubles during production and boost item efficiency.
5. Efficient after-sales service
Zhishang Bio provides complete monitoring, reacts to client requests and delivers promptly. This will certainly make certain customer complete satisfaction.
Zhishang Biography intends to help consumers expand their service by providing the very best solutions and chemical resources.
FAQ
DO YOU ACCEPT SAMPLE ORDER?
We will make samples before mass production. After the samples are approved by the department and users, we will start mass production. During the production process, we will carry out 100% inspection, and then carry out random inspection before packaging.
WHAT’S YOUR MOQ?
Our MOQ is 1kg. But usually we can also accept a smaller quantity, such as 100g, if you need it, provided that the sample fee is 100% paid.
IS THERE A DISCOUNT?
Different quantity has different discount.
HOW TO CONFIRM WHETHER THE PRODUCT QUALITY MEETS OUR NEEDS BEFORE PLACING AN ORDER?
You can consult us to get free samples of some products, you only need to pay the freight or arrange express delivery to us and get the samples. You can send us the product specifications and requirements you need, and we will manufacture products according to your requirements.
DO YOU SUPPLY PRODUCT REPORT?
Yes. We’ll give you product analysis report before shipping.
LEARN MORE ABOUT US
Shandong Look Chemical
Advice on Shipping
1. For products ≤50kg, we recommend using express delivery, which is usually called DDU service (discounted, convenient).
2. For products ≤500kg, we generally recommend air freight, which is usually called FOB, CFR or CIF service (fast and efficient).
3. For products >500kg, we generally recommend shipping by sea, which is usually called FOB, CFR or CIF service (economical, safe).
4. For high-value products, please choose air or express to ensure the safety of product transportation.
Company Info
Zhishang Bio company in China that exports chemicals, Cosmetic raw materials, and Intermediates for over 70 countries now.
And a range of 50,000 products with the best quality and competitive price to meet your different purchasing needs.
The company integrates research and development, production, and trade, with a modern production base, advanced testing equipment, and an experienced technical team to ensure that products reach industry-leading levels in purity, stability, and environmental friendliness.
