Fluorescein Isothiocyanate Isomer I | High Purity Biochemical Reagent
Fluorescein isothiocyanate isomer I CAS 3326-32-7
- Surfactant Appearance: powder
- Proportion: Minimum 99.0%
- Inventory: In stock
- Sample: In stock
- Zhishang Chemical: Supplier and manufacturer of Fluorescein isothiocyanate isomer I
Basic Info of Fluorescein isothiocyanate isomer I
| Fluorescein isothiocyanate isomer I Chemical Properties |
| Melting point | >360 °C(lit.) |
| Boiling point | 708.6±60.0 °C(Predicted) |
| density | 1.3423 (rough estimate) |
| refractive index | 1.5060 (estimate) |
| storage temp. | 2-8°C |
| solubility | Slightly soluble in DMSO, ethanol, methanol DMF and acetone |
| pka | 2.2, 4.4, 6.7(at 25℃) |
| form | powder |
| color | White |
| Appearance | Yellow solid |
| PH Range | Weak green uorescence (6.0) to strong green uorescence (7.2) |
| Water Solubility | practically insoluble |
| ε(extinction coefficient) | 74,000 L⋅mol−1⋅cm−1 |
What is Fluorescein isothiocyanate isomer I ？
Fluorescein isothiocyanate isomer I, commonly abbreviated as FITC (Isomer I), has the CAS number 3326-32-7. It is a classic amine-reactive green fluorescent color. The term “isomer I” in its name specifically describes the isothiocyanate team attached to the 5th placement of the bottom benzene ring of the fluorescein molecule (5-FITC), in contrast to the isomer II where the isothiocyanate team is attached to the 6th position.
The core design of FITC depends on its isothiocyanate group. Fluorescein isothiocyanate isomer I can undergo certain covalent reactions with proteins, antibodies, or amino teams on polypeptide chains under alkaline problems, forming stable thiourea bonds. In regards to photophysical residential or commercial properties, the maximum excitation wavelength (Ex lover) of FITC is about 492 nm, and the optimum exhaust wavelength (Em) is around 518-520 nm, providing a typical yellow-green fluorescence.
The solid powder of fluorescein isothiocyanate isomer I is usually orange-yellow in color, with a melting point higher than 360 ° C and having hygroscopic buildings. Fluorescein isothiocyanate isomer I is sensitive to pH modifications, and its fluorescence intensity significantly raises in alkaline atmospheres, while it weakens or even vanishes in acidic problems.
In the field of life science research, Fluorescein isothiocyanate isomer I is one of the most commonly made use of fluorescent labeling reagents.
( 1) Healthy protein and antibody labeling: This is the core application of Fluorescein isothiocyanate isomer I. With an easy chemical reaction, fluorescent particles can be covalently attached to antibodies, which can be used for immunofluorescence detection, flow cytometry, and protein mapping.
( 2) Rapid medical diagnosis of pathogens: By using FITC-labeled certain antibodies, one can straight identify infections or microorganisms in tissue sections or examples, which is a common approach for fast clinical pathological diagnosis.
( 3) Nucleic acids and cell chemistry: It can be utilized to identify lectins or specific nucleic acid probes, assisting in the research of cell membrane layer structure or gene localization.
Product Packaging
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Superior Fluorescein isothiocyanate isomer I Series | Reliable Quality & Sufficient Supply
Fill Out and Purchase Recommendations
As an expert provider of Fluorescein isothiocyanate isomer I, we are aware of the stringent requirements of clinical research users for the security and labeling performance of reagents. In regards to supply, we provide a selection of items with pureness varying from ≥ 90% to ≥ 97.5%, all of which have been purely checked by high-performance fluid chromatography to make certain the material and set security of Fluorescein isothiocyanate isomer I.
Due to the fact that the isothiocyanate groups are very vulnerable to hydrolysis and inactivation, the Fluorescein isothiocyanate isomer I produced by us is packaged under argon gas and sealed. It is advised that the whole process be accomplished under -20 ° C for suspended animation, and afterwards transferred and stored in a dark atmosphere. The routine in-stock requirements sustain packaging of 100mg, 500mg and 1g, which can satisfy various requirements ranging from fundamental research to the production of analysis kits.
For healthy protein labeling experiments, we can supply a dedicated supply of anhydrous dimethyl sulfoxide solvent and thorough labeling buffer remedies. This will certainly aid users prevent interference from cost-free amines and significantly boost the combining performance.
FAQ
Because the active group isothiocyanate of FITC is easily hydrolyzed and deactivated, solid powders will become ineffective if they absorb moisture, making them more unstable in solution. Unopened solid powders can be stored stably for at least 12-24 months under dry and dark conditions at -20 ° C; Once prepared into dimethyl sulfoxide storage solution, it is recommended to store it in the dark at -20 ° C after packaging and use it up within one month.
Definitely. COA, MSDS and other necessary certificates are all available.
We use professional sealed packaging. Custom packaging is supported as requested.
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Fluorescein isothiocyanate isomer I Supplier and Manufacturer
As a Fluorescein isothiocyanate isomer I supplier and manufacturer with a strict standard product quality system certificate, Zhishang Chemical has long been providing the best Fluorescein isothiocyanate isomer I raw materials to customers all over the world.
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