PRODUCT
Description
1. Chemical properties and classification
Methylene Blue trihydrate， Methylene Blue trihydrate is a phenothiazine cationic dye with the chemical formula C ₁₆ H ₁₈ ClN ∝ S · 3H ₂ O, a molecular weight of 373.90, and a CAS number of 7220-79-3. Its molecular structure is centered around the phenothiazine ring, with one dimethylamino group at each of the 3rd and 7th positions. The central sulfur atom carries a positive charge and forms a salt with chloride ions. Trihydrate is its most stable crystalline form, containing three crystal waters, which is also the most common form in laboratories and industry.
2. Physical characteristics and morphology
At room temperature, Methylene Blue trihydrate is a shiny dark green crystal or small dark brown powder with a bronze luster and no odor. Its melting point is about 190 ° C (decomposition) and its density is about 0.98 g/cm ³. This compound is soluble in water and chloroform, soluble in ethanol (slightly soluble), and appears sky blue in aqueous solution. It is insoluble in ether and benzene. The pH value of a 1% aqueous solution is about 3, with characteristic absorption peaks at 668 nm and 609 nm.
3. Discovery process and industry positioning
Methylene Blue trihydrate was first synthesized by German chemist Heinrich Caro at BASF in 1876 and was initially used as a dye for cotton textiles. In 1886, German scientist Paul Ehrlich discovered that it could selectively stain neural tissue, opening up its application in the field of biomedical science
In 1891, Ehrlich and Guttmann used it for the treatment of human malaria, making it the first synthetic antimalarial drug. Thus, Methylene Blue trihydrate has completed its transformation from an industrial dye to a medical reagent. With its redox properties and staining ability, it occupies an important position in multiple fields such as biological staining, analytical chemistry, and clinical treatment.
Product Images & Packaging
Methylene Blue trihydrate Parameters
| Methylene Blue trihydrate Chemical Properties |
| Melting point | 190 °C (dec.)(lit.) |
| bulk density | 400-600kg/m3 |
| density | 0.98 g/mL at 25 °C |
| Fp | 14 °C |
| storage temp. | no restrictions. |
| solubility | Soluble in water, chloroform; sparingly soluble in ethanol |
| pka | 2.6, 11.2(at 25℃) |
| Colour Index | 52015 |
| form | Solid |
| color | Green |
| PH | 3 (20°C, 10g/L in H2O) |
| Water Solubility | 4 g/100 mL |
| λmax | 668 nm, 609 nm |
| Merck | 13,6085 |
The application of Methylene Blue trihydrate
The main uses of Methylene Blue trihydrate
Methylene Blue trihydrate, as a classic phenothiazine compound, plays an irreplaceable role in various fields such as biological staining, analytical chemistry, clinical medicine, and scientific research due to its unique redox properties and staining ability
Biological staining and histology field
Methylene Blue trihydrate is one of the most commonly used live dyes and contrast dyes in biology and medical laboratories. It can color the nucleus and certain organelles, making it easier to observe cell morphology and structure under a microscope. In microbiology, it is commonly used for simple staining of bacteria and can also be used as a counterstain in the Ziehl Neelsen acid fast staining method to stain non acid fast bacteria blue. In histology, it is an important component of many staining solutions such as Wright staining and Giemsa staining, used for staining blood smears and assisting in blood cell classification and counting. In cell viability testing, live cells can reduce Methylene Blue trihydrate to colorless reduced form (methylene white), while dead cells cannot. This characteristic makes it an effective tool for evaluating cell survival rate.
In the field of analytical chemistry
The redox properties of Methylene Blue trihydrate make it of significant value in analytical chemistry. It is used as an oxidation-reduction indicator in titration analysis to indicate the endpoint of titration, and the completion of the reaction is judged by the change in solution color (blue to colorless). It can also be used to detect and determine the content of sulfides in water, and perform semi quantitative or quantitative analysis through colorimetric reactions. In addition, in the classic “blue bottle experiment”, Methylene Blue trihydrate was reduced and faded in alkaline glucose solution, and then regained its blue color after oscillation. This reversible color change vividly demonstrates the dynamic equilibrium of redox reactions and is commonly used as a demonstration experiment in chemistry teaching. In photometric analysis, Methylene Blue trihydrate can also be used as a chromogenic reagent for colorimetric determination of various metal ions such as arsenic, gold, boron, iridium, etc.
Clinical medicine field: Detoxification drugs and diagnostic tools
In clinical medicine, the most classic application of Methylene Blue trihydrate is the treatment of methemoglobinemia. When the human body ingests substances such as nitrite and nitrobenzene, the iron in hemoglobin is oxidized to trivalent, losing its oxygen carrying capacity and leading to tissue hypoxia. Methylene Blue trihydrate can act as an electron donor, reducing trivalent iron to divalent iron, restoring the oxygen carrying function of hemoglobin, and thus playing a detoxifying role. It is also used as an adjuvant therapy for cyanide poisoning. In addition, Methylene Blue trihydrate has bactericidal and antifungal activity and can be used to treat cystitis and urethritis (by oral administration or flushing). In ornamental fish farming, 0.1-0.2 ppm Methylene Blue trihydrate solution is widely used for water disinfection and treatment of parasitic infections such as white spot disease in fish. Due to the fact that Methylene Blue trihydrate is excreted from urine within a few hours after oral administration and turns the urine blue, it has also been used as an indicator for renal function testing.
Scientific research and cutting-edge fields
In modern scientific research, Methylene Blue trihydrate has shown broader application prospects. In the field of neuroscience, research has found that low-dose Methylene Blue trihydrate can improve mitochondrial function, enhance energy metabolism in brain cells, and have neuroprotective effects. Currently, its potential in treating neurodegenerative diseases such as Alzheimer’s disease is being studied. In the field of tumor treatment, Methylene Blue trihydrate is used as a photosensitizer for photodynamic therapy, which can produce reactive oxygen species under specific wavelength light irradiation and selectively kill tumor cells. In the fields of antibacterial research and industry, Methylene Blue trihydrate is used to develop disinfectants and preservatives due to its inhibitory effect on various bacteria and fungi. In addition, it can also be used in the manufacturing of ink and color lakes, as well as in coloring processes for materials such as bamboo and wood.
The use of Methylene Blue trihydrate fully demonstrates the value of the compound’s “multifunctionality”. It is not only a staining tool for observing life under a microscope, but also a detoxifying medicine for rescuing poisoned patients; It is not only a color sentinel that indicates the endpoint in chemical experiments, but also a research tool for combating cancer and neurodegenerative diseases in cutting-edge science. This classic compound, with a history of nearly 150 years, continues to contribute new value in multiple fields with the support of modern science and technology.
Methylene Blue trihydrate 's Procurement Guide
Procurement Guidelines
1. Sample Policy
We can provide a 2-3g free trial package (shipping fee required)
2. Batch procurement
Packaging: 25kg/bag; 220kg/barrel; Special packaging according to your needs
MOQ：1kg
3. Customized services
Concentration customization (90% -99%)
Paeonol’s outsourcing services
FAQ
Trihydrate is preferred for daily laboratory staining, water quality testing, and aquaculture. It has better stability, is less likely to absorb moisture, and has a more stable color in aqueous solutions; Anhydrous type is commonly used for precision organic synthesis and high standard solution preparation.
Methylene Blue trihydrate can be used for simple bacterial staining, live cell staining, redox titration indicators, rapid detection of dissolved oxygen/ammonia nitrogen in water, and is also a commonly used staining reagent in biology teaching and research laboratories.
It has excellent water solubility and can be quickly dissolved in clean water at room temperature to form a clear blue solution without precipitation or layering. It is suitable for long-term storage of standard staining solutions and testing working solutions.
Methylene Blue trihydrate should be sealed, protected from light, stored in a cool and dry place, and kept away from strong sunlight and humid environments; Normal storage has a long shelf life and is prone to fading and reduced dyeing efficiency when exposed to strong light. It is recommended to store in a dark container.
Related References
Manufacturer of Methylene Blue trihydrate
Achievements
Advantage
Service
FAQ
Advice on Shipping
Achievements
Zhishang Bio is based in Shandong, serves the whole country, faces the world, and is determined to become an excellent supplier and service provider in the chemical industry.
As a top manufacturer of cosmetic raw materials, disinfectants, flavors and fragrances in China, we are committed to producing high-quality products and have won high praise from customers all over the world.
In the next 10 years or even longer, Zhishang Bio will always adhere to the tenet of quality first and reputation first, and devote itself to providing customers with thoughtful and perfect services.
As a professional Methylene Blue trihydrate manufacturer, Zhi shang Bio has served more than 6,000 customers around the world. Because of our high-quality products and professional services, we have won high praise from most of our customers.
Advantage
Zhishang Bio is a professional enterprise engaged in the research and development, production, and sales of chemical raw materials. It has a strong supply chain system and strict quality control standards to ensure the provision of high-quality products with high purity and stability to customers.
Zhishang Bio’s core strength is its independent research and design capabilities. It has advanced production equipment, testing laboratories and can customize chemical raw materials to meet the market demands. This includes pharmaceuticals, electronic, coatings and agricultural applications.
Zhishang Bio’s supply chain management is based on long-term relationships with domestic and international suppliers of high quality to guarantee a stable supply, optimize logistic systems, and achieve efficient distribution. This helps reduce the cost of customer purchases.
We adhere to international environmental standards and we also adhere to the concept that environmental protection and safety production are important. All production processes must comply with these standards in order to guarantee that our products will be green and sustainable.
With a professional technical team and comprehensive after-sales service, Zhishang Bio can provide customers with precise technical support, solve application problems, and help customers enhance product competitiveness.
We are always customer-oriented and committed to becoming a trusted chemical raw material partner.
Service
Zhishang Biography is dedicated to using clients extensive and expert service, including chemical raw material. The customers will certainly receive hassle-free, efficient and one-stop options.
1. Custom-made Supply Solutions
Zhishang Bio provides tailored services for clients in the chemical basic material sector, including special bit dimension, solvent residue control, stability optimizations and more. Clients can personalize their services to satisfy details requirements in industries such as drugs, electronic devices, and finishings.
2. Strict quality assurance
Zhishang Bio utilizes advanced screening equipment and stringent quality examination procedures, in addition to various other files, to guarantee that every batch of products is certified with international criteria such ISO GMP, REACH, and so on. Customers are also given with full quality records and conformity documents.
The supply chain is secure
Zhishang Bio has actually established lasting calculated collaborations with worldwide makers of top notch raw materials to make certain a secure supply, maximize the storage facility and logistics system, and assistance both small-scale examinations and large orders. It adjusted to the changing requirements of clients by responding flexibly to these changes.
4. Technical support
Zhishang Bio’s technical group can offer suggestions on product applications, formula optimization and analysis of industry patterns to aid clients fix technological troubles during production and boost item efficiency.
5. Efficient after-sales service
Zhishang Bio provides complete monitoring, reacts to client requests and delivers promptly. This will certainly make certain customer complete satisfaction.
Zhishang Biography intends to help consumers expand their service by providing the very best solutions and chemical resources.
FAQ
DO YOU ACCEPT SAMPLE ORDER?
We will make samples before mass production. After the samples are approved by the department and users, we will start mass production. During the production process, we will carry out 100% inspection, and then carry out random inspection before packaging.
WHAT’S YOUR MOQ?
Our MOQ is 1kg. But usually we can also accept a smaller quantity, such as 100g, if you need it, provided that the sample fee is 100% paid.
IS THERE A DISCOUNT?
Different quantity has different discount.
HOW TO CONFIRM WHETHER THE PRODUCT QUALITY MEETS OUR NEEDS BEFORE PLACING AN ORDER?
You can consult us to get free samples of some products, you only need to pay the freight or arrange express delivery to us and get the samples. You can send us the product specifications and requirements you need, and we will manufacture products according to your requirements.
DO YOU SUPPLY PRODUCT REPORT?
Yes. We’ll give you product analysis report before shipping.
LEARN MORE ABOUT US
Shandong Look Chemical
Advice on Shipping
1. For products ≤50kg, we recommend using express delivery, which is usually called DDU service (discounted, convenient).
2. For products ≤500kg, we generally recommend air freight, which is usually called FOB, CFR or CIF service (fast and efficient).
3. For products >500kg, we generally recommend shipping by sea, which is usually called FOB, CFR or CIF service (economical, safe).
4. For high-value products, please choose air or express to ensure the safety of product transportation.
Company Info
Zhishang Bio company in China that exports chemicals, Cosmetic raw materials, and Intermediates for over 70 countries now.
And a range of 50,000 products with the best quality and competitive price to meet your different purchasing needs.
The company integrates research and development, production, and trade, with a modern production base, advanced testing equipment, and an experienced technical team to ensure that products reach industry-leading levels in purity, stability, and environmental friendliness.
