PRODUCT
Neutral Red CAS 553-24-2
CAS:553-24-2 | C15H17ClN4 |MW：288.78 | EINECS NO：209-035-8
Payment: L/C,T/T,D/P,Paypal,Money Gram,Western Union
Incoterm: FOB,CFR,CIF,EXW,FCA,CPT,CIP
Transporta: Ocean, Land,Air, DHL，TNT
Port: Shanghai Port, Qingdao Port
Description
This is an introduction to Neutral Red. Neutral Red, The chemical name is 3-amino-7-dimethylamino-2-methylphenazine hydrochloride, which is an important phenazine alkaline dye and redox indicator. The tricyclic mother nucleus is the core of this dye. It’s connected to chromophores like amino and dimethylamino. Physical and chemical properties Neutral Red is usually a dark-green crystalline powder that has a metallic sheen, and it can also be found as its salt. It is moderately soluble in water, and also soluble in polar solvants such as ethanol and water. Its most distinctive feature is that the color of the solution changes significantly and reversibly with pH values. Neutral Red is a neutral red that changes color between pH 6.8 to pH 8.0. It appears orange-yellow under alkaline and red in acidic conditions. The protonation/deprotonation of the dimethylamino group in its molecules is responsible for this discoloration. It also exhibits certain fluorescence properties. Chemical property Neutral Red, as an oxidation reduction indicator, can undergo reversible reactions of oxidation and reduction in biological systems. It has a red state for oxidation, followed by a colorless state for the reduction. The phenazine-structure of Neutral Red allows it to accept and give electrons in a reversible manner. It is used in biology as a “living color” or “hyperactive colour” which can pass through intact cell membranes, enter organelles and accumulate in acidic environments. The following are the key characteristics and status. Neutral Red’s unique combination of low cytotoxicity and membrane permeability is its core value. It is a versatile dye that can be used as an acid-base indicator, and also as a probe for interacting with living cells. Its synthesis usually involves oxidative condensing of nitrotoluene derivatives.
Product Images & Packaging
Product Parameters
| Neutral Red Chemical Properties |
| Melting point | 290 °C (dec.) (lit.) |
| Boiling point | 441.31°C (rough estimate) |
| bulk density | 350-500kg/m3 |
| density | 1.1590 (rough estimate) |
| refractive index | 1.6110 (estimate) |
| storage temp. | Store at +5°C to +30°C. |
| solubility | H2O: soluble10mg/mL |
| pka | 6.7, 7.4(at 25℃) |
| form | Solid |
| Colour Index | 50040 |
| color | Very dark green |
| Appearance | Solid Powder |
| Odor | Odorless |
| PH | 3.1 (10g/l, H2O, 20℃) |
| PH Range | 6.8(red)-8(yellow) |
| Water Solubility | 50 g/L |
| λmax | 540nm, 533nm, 544nm, 529nm, 454nm |
The application of Neutral Red
1.Applications in biology and medical research
Neutral Red is a unique dye that can be used to detect cells and live organisms. Its transmembrane characteristics and its specific response in acidic environments make it an indispensable tool for life science research.
The classic method for the detection of cell viability: Neutral Red Assay is one gold standard method to evaluate cell viability.
Neutral Red can only be actively stored by lysosomes of living cells. The proportion of living cells can be determined by measuring the amount dye taken up in the cells. This is important for drug screenings, toxicology studies, and biomaterial compatibility tests.
Labeling specific lysosome functions: Neutral Red, a fluorescent marker for acidic organelles, is widely used to label lysosomes. This is due to the fact that it selectively enriches and fluoresces in these organelles. Researchers use this characteristic to observe quantity, size and distribution of lysosomes, as well as dynamic changes in lysosomes, when imaging live cells. They also study pathological processes, such autophagy and endocytosis.
Neutral Red is a staining agent that can be used in traditional histology, microbiology and to analyze pathogens and tissues. In virology it can be used to stain live cells in order to display viral plaques in experiments. In parasitology it can be used as a way to differentiate between dead and live parasites.
2. Applications in the field analytical chemistry
Neutral Red, with its sensitive color and fluorescence reaction, is a useful tool in analytical chemistry.
Acid base indicator: Its wide range of color changes makes it a great indicator for monitoring pH changes in the environment or physiologically.
Redox indicator. Neutral Red is a reversible color-changing reaction that can be used in redox titration to indicate potential endpoints.
Chemical sensors to detect metal ions: The nitrogen and oxygen atoms of Neutral Red can act as coordination site and bind certain metal ions. This causes significant changes in the absorption and fluorescence spectrum.
This principle can be used to build colorimetric or fluorescent sensors that are high-sensitivity, low-cost and capable of detecting certain metal ions within water or biological samples.
3.Environmental Science and Toxicology Monitor
The characteristics of the probe as a biological sensor have been extended to environmental assessment.
Biomarkers for Environmental Toxicity – Conducting Neutral Red Uptake Experiments using Fish Cell Lines or directly using Small Aquatic Organisms is a reliable and rapid biological test method to evaluate acute toxicity in water bodies, sediments or chemicals.
Assessing the biological effects of pollution exposure: The uptake of Neutral Red in biological cells can be used to quantify the damage that pollutants have caused to the cell membrane integrity, stability of lysosomes, and metabolic activity.
4.Industrial and Materials Science
Even though the scale of application is small, its unique characteristics play a part in certain industrial processes.
Textile dyeing. In the past, Neutral Red, an alkaline color, was used to dye protein fibers, such as wool and silk, resulting in a vibrant red hue.
Functional material preparation : Its redox activities have been studied to prepare certain electrochromic materials, or as an electron-transfer medium integrated into sensing films.
Neutral Red's Procurement Guide
Procurement Guidelines
1. Sample Policy
We can provide a 2-3g free trial package (shipping fee required)
2. Batch procurement
Packaging: 25kg/bag; 220kg/barrel; Special packaging according to your needs
MOQ：1kg
3. Customized services
Concentration customization (90% -99%)
Paeonol’s outsourcing services
FAQ
Yes, we have our own factory called ” ZhiShang Industry Group “
Yes, we have GMP certificate for some products, and we can provide DMF as well.
-Quality you require, eg. Assay, Purity, or single impurity
-Quantity you need
-Standard you want, such as USP, EP, JP etc.
Escrow and T/T in advance are highly recommended. We can accept Western Union, Money Gram and Paypal as well.
For sample(less than 1kg), in about 3 working days after payment; for larger orders (more than 1kg), in about 7 working days after payment.
For samples, we will send by TNT/DHL/FEDEX/UPS express, usually it takes about 3-5 day.
Related References
Manufacturer of Neutral Red
Achievements
Advantage
Service
FAQ
Advice on Shipping
Achievements
Zhishang Bio is based in Shandong, serves the whole country, faces the world, and is determined to become an excellent supplier and service provider in the chemical industry.
As a top manufacturer of cosmetic raw materials, disinfectants, flavors and fragrances in China, we are committed to producing high-quality products and have won high praise from customers all over the world.
In the next 10 years or even longer, Zhishang Bio will always adhere to the tenet of quality first and reputation first, and devote itself to providing customers with thoughtful and perfect services.
As a professional Neutral Red manufacturer, Zhi shang Bio has served more than 6,000 customers around the world. Because of our high-quality products and professional services, we have won high praise from most of our customers.
Advantage
Zhishang Bio is a professional enterprise engaged in the research and development, production, and sales of chemical raw materials. It has a strong supply chain system and strict quality control standards to ensure the provision of high-quality products with high purity and stability to customers.
Zhishang Bio’s core strength is its independent research and design capabilities. It has advanced production equipment, testing laboratories and can customize chemical raw materials to meet the market demands. This includes pharmaceuticals, electronic, coatings and agricultural applications.
Zhishang Bio’s supply chain management is based on long-term relationships with domestic and international suppliers of high quality to guarantee a stable supply, optimize logistic systems, and achieve efficient distribution. This helps reduce the cost of customer purchases.
We adhere to international environmental standards and we also adhere to the concept that environmental protection and safety production are important. All production processes must comply with these standards in order to guarantee that our products will be green and sustainable.
With a professional technical team and comprehensive after-sales service, Zhishang Bio can provide customers with precise technical support, solve application problems, and help customers enhance product competitiveness.
We are always customer-oriented and committed to becoming a trusted chemical raw material partner.
Service
Zhishang Biography is dedicated to using clients extensive and expert service, including chemical raw material. The customers will certainly receive hassle-free, efficient and one-stop options.
1. Custom-made Supply Solutions
Zhishang Bio provides tailored services for clients in the chemical basic material sector, including special bit dimension, solvent residue control, stability optimizations and more. Clients can personalize their services to satisfy details requirements in industries such as drugs, electronic devices, and finishings.
2. Strict quality assurance
Zhishang Bio utilizes advanced screening equipment and stringent quality examination procedures, in addition to various other files, to guarantee that every batch of products is certified with international criteria such ISO GMP, REACH, and so on. Customers are also given with full quality records and conformity documents.
The supply chain is secure
Zhishang Bio has actually established lasting calculated collaborations with worldwide makers of top notch raw materials to make certain a secure supply, maximize the storage facility and logistics system, and assistance both small-scale examinations and large orders. It adjusted to the changing requirements of clients by responding flexibly to these changes.
4. Technical support
Zhishang Bio’s technical group can offer suggestions on product applications, formula optimization and analysis of industry patterns to aid clients fix technological troubles during production and boost item efficiency.
5. Efficient after-sales service
Zhishang Bio provides complete monitoring, reacts to client requests and delivers promptly. This will certainly make certain customer complete satisfaction.
Zhishang Biography intends to help consumers expand their service by providing the very best solutions and chemical resources.
FAQ
DO YOU ACCEPT SAMPLE ORDER?
We will make samples before mass production. After the samples are approved by the department and users, we will start mass production. During the production process, we will carry out 100% inspection, and then carry out random inspection before packaging.
WHAT’S YOUR MOQ?
Our MOQ is 1kg. But usually we can also accept a smaller quantity, such as 100g, if you need it, provided that the sample fee is 100% paid.
IS THERE A DISCOUNT?
Different quantity has different discount.
HOW TO CONFIRM WHETHER THE PRODUCT QUALITY MEETS OUR NEEDS BEFORE PLACING AN ORDER?
You can consult us to get free samples of some products, you only need to pay the freight or arrange express delivery to us and get the samples. You can send us the product specifications and requirements you need, and we will manufacture products according to your requirements.
DO YOU SUPPLY PRODUCT REPORT?
Yes. We’ll give you product analysis report before shipping.
LEARN MORE ABOUT US
Shandong Look Chemical
Advice on Shipping
1. For products ≤50kg, we recommend using express delivery, which is usually called DDU service (discounted, convenient).
2. For products ≤500kg, we generally recommend air freight, which is usually called FOB, CFR or CIF service (fast and efficient).
3. For products >500kg, we generally recommend shipping by sea, which is usually called FOB, CFR or CIF service (economical, safe).
4. For high-value products, please choose air or express to ensure the safety of product transportation.
Company Info
Zhishang Bio company in China that exports chemicals, Cosmetic raw materials, and Intermediates for over 70 countries now.
And a range of 50,000 products with the best quality and competitive price to meet your different purchasing needs.
The company integrates research and development, production, and trade, with a modern production base, advanced testing equipment, and an experienced technical team to ensure that products reach industry-leading levels in purity, stability, and environmental friendliness.
