PRODUCT
Zhishang Bio 3 3-Diaminobenzidine CAS 91-95-2
CAS:91-95-2 | C12H14N4 |MW：214.27 | EINECS NO：202-110-6
Payment: L/C,T/T,D/P,Paypal,Money Gram,Western Union
Incoterm: FOB,CFR,CIF,EXW,FCA,CPT,CIP
Transporta: Ocean, Land,Air, DHL，TNT
Port: Shanghai Port, Qingdao Port
Description
3 3-Diaminobenzidine is a volatile fermentation product that is widely used as a food additive due to its unique aroma.
3 3-Diaminobenzidine has significant biochemical and toxicological properties, and its pleasant aroma may make people overlook its inherent harm to human health.
In addition, 3 3-Diaminobenzidine has a wide range of applications in various research fields, mainly as a monomer for the preparation of high-temperature resistant resins and fiber materials. However, its application in the field of analysis is not common, mainly used for the determination of selenium content by spectrophotometry.
The molecular formula of 3 3-Diaminobenzidine is C ₁₂ H ₁₄ N ₄ · 4HCl, with a molecular weight of 360.18 g/mol
Physical properties of 3 3-Diaminobenzidine:
Usually a crystalline powder ranging from gray white to light brown or dark brown in color. Easy to dissolve in water, forming a colorless or light yellow solution, but the color will undergo a drastic change after oxidation.
Chemical properties of 3 3-Diaminobenzidine:
The core chemical properties of DAB are its strong reducibility and ability to act as a chromophore.
Under the catalysis of peroxidase, 3 3-Diaminobenzidine is oxidized by hydrogen peroxide and undergoes polymerization reaction, producing an insoluble, brownish polymer. This color reaction is its most famous and widely used characteristic.
Product Images & Packaging
Product Parameters
| 3 3-Diaminobenzidine Chemical Properties |
| Melting point | 175-177 °C(lit.) |
| Boiling point | 344.41°C (rough estimate) |
| density | 1.1726 (rough estimate) |
| refractive index | 1.5000 (estimate) |
| Fp | 12 °C |
| storage temp. | room temp |
| solubility | 0.55g/l |
| form | tablet |
| pka | 4.39±0.10(Predicted) |
| color | Crystals from MeOH |
| PH | 7 (1g/l, H2O, 20℃) |
| Water Solubility | 0.55 g/L (20 ºC) |
| Sensitive | Light Sensitive |
| BRN | 1212988 |
| Stability: | Moisture and light sensitive. Incompatible with strong oxidizing agents. |
| InChIKey | HSTOKWSFWGCZMH-UHFFFAOYSA-N |
The application of 3 3-Diaminobenzidine
3 3-Diaminobenzidine is an essential chromogenic substrate in biomedical research. All of its applications are based on a core biochemical reaction: catalyzed by horseradish peroxidase, it is oxidized by hydrogen peroxide to form insoluble brown polymer precipitates. This feature makes it a classic tool for in-situ detection and target localization.
In the fields of immunohistochemistry and immunohistochemistry, 3 3-Diaminobenzidine plays a crucial role. After binding to the target antigen with specific antibodies, HRP labeled secondary antibodies catalyze the colorimetric reaction of 3-Diaminobenzidine, forming a stable brown precipitate at the location of the antigen.
This precipitate is insoluble in water and organic solvents, allowing for long-term preservation of staining results and forming a sharp contrast with counterstaining agents such as hematoxylin, providing permanent records for protein localization under optical microscopy.
For Western blot analysis, 3 3-Diaminobenzidine was one of the earliest colorimetric methods used. Although chemiluminescence has become mainstream due to its higher sensitivity, the 3 3-Diaminobenzidine colorimetric method is still retained in some laboratories for rapid detection due to its low cost, simple operation, and intuitive results. Researchers can directly observe the brown bands appearing on the membrane without the need for special imaging equipment.
In enzyme-linked immunosorbent assay, 3 3-Diaminobenzidine plays an important role as a chromogenic substrate for HRP. After completing the antigen antibody reaction, adding the substrate solution of 3 3-Diaminobenzidine will cause a color change. Quantitative analysis of the target molecule can be achieved by measuring the absorbance value using an enzyme-linked immunosorbent assay (ELISA) reader. This method is suitable for the routine testing needs of most laboratories.
The application of 3 3-Diaminobenzidine has also expanded to the field of nucleic acid research. In situ hybridization (ISH) experiments, by hybridizing biotin or digoxin labeled nucleic acid probes with target sequences and using the HRP-DAB colorimetric system, the expression positions of specific genes or mRNA transcripts can be displayed in situ in tissues, providing important information for gene expression research.
The most noteworthy is the unique application of 3 3-Diaminobenzidine in the field of electron microscopy. The 3 3-Diaminobenzidine oxidized polymer has electron density characteristics, and after fixation with osmium acid, its electron scattering ability can be further enhanced. This enables researchers to accurately locate antigens at the ultrastructural level, providing a reliable method for studying protein distribution at the organelle and membrane levels.
3 3-Diaminobenzidine's Procurement Guide
Procurement Guidelines
1. Sample Policy
We can provide a 2-3g free trial package (shipping fee required)
2. Batch procurement
Packaging: 25kg/bag; 220kg/barrel; Special packaging according to your needs
MOQ：1kg
3. Customized services
Concentration customization (90% -99%)
Paeonol’s outsourcing services
FAQ
Yes, we have our own factory called ” ZhiShang Industry Group “
Yes, we have GMP certificate for some products, and we can provide DMF as well.
-Quality you require, eg. Assay, Purity, or single impurity
-Quantity you need
-Standard you want, such as USP, EP, JP etc.
Escrow and T/T in advance are highly recommended. We can accept Western Union, Money Gram and Paypal as well.
For sample(less than 1kg), in about 3 working days after payment; for larger orders (more than 1kg), in about 7 working days after payment.
For samples, we will send by TNT/DHL/FEDEX/UPS express, usually it takes about 3-5 day.
Related References
Manufacturer of 3 3-Diaminobenzidine
Achievements
Advantage
Service
FAQ
Advice on Shipping
Achievements
Zhishang Bio is based in Shandong, serves the whole country, faces the world, and is determined to become an excellent supplier and service provider in the chemical industry.
As a top manufacturer of cosmetic raw materials, disinfectants, flavors and fragrances in China, we are committed to producing high-quality products and have won high praise from customers all over the world.
In the next 10 years or even longer, Zhishang Bio will always adhere to the tenet of quality first and reputation first, and devote itself to providing customers with thoughtful and perfect services.
As a professional 3 3-Diaminobenzidine manufacturer, Zhi shang Bio has served more than 6,000 customers around the world. Because of our high-quality products and professional services, we have won high praise from most of our customers.
Advantage
Zhishang Bio is a professional enterprise engaged in the research and development, production, and sales of chemical raw materials. It has a strong supply chain system and strict quality control standards to ensure the provision of high-quality products with high purity and stability to customers.
Zhishang Bio’s core strength is its independent research and design capabilities. It has advanced production equipment, testing laboratories and can customize chemical raw materials to meet the market demands. This includes pharmaceuticals, electronic, coatings and agricultural applications.
Zhishang Bio’s supply chain management is based on long-term relationships with domestic and international suppliers of high quality to guarantee a stable supply, optimize logistic systems, and achieve efficient distribution. This helps reduce the cost of customer purchases.
We adhere to international environmental standards and we also adhere to the concept that environmental protection and safety production are important. All production processes must comply with these standards in order to guarantee that our products will be green and sustainable.
With a professional technical team and comprehensive after-sales service, Zhishang Bio can provide customers with precise technical support, solve application problems, and help customers enhance product competitiveness.
We are always customer-oriented and committed to becoming a trusted chemical raw material partner.
Service
Zhishang Biography is dedicated to using clients extensive and expert service, including chemical raw material. The customers will certainly receive hassle-free, efficient and one-stop options.
1. Custom-made Supply Solutions
Zhishang Bio provides tailored services for clients in the chemical basic material sector, including special bit dimension, solvent residue control, stability optimizations and more. Clients can personalize their services to satisfy details requirements in industries such as drugs, electronic devices, and finishings.
2. Strict quality assurance
Zhishang Bio utilizes advanced screening equipment and stringent quality examination procedures, in addition to various other files, to guarantee that every batch of products is certified with international criteria such ISO GMP, REACH, and so on. Customers are also given with full quality records and conformity documents.
The supply chain is secure
Zhishang Bio has actually established lasting calculated collaborations with worldwide makers of top notch raw materials to make certain a secure supply, maximize the storage facility and logistics system, and assistance both small-scale examinations and large orders. It adjusted to the changing requirements of clients by responding flexibly to these changes.
4. Technical support
Zhishang Bio’s technical group can offer suggestions on product applications, formula optimization and analysis of industry patterns to aid clients fix technological troubles during production and boost item efficiency.
5. Efficient after-sales service
Zhishang Bio provides complete monitoring, reacts to client requests and delivers promptly. This will certainly make certain customer complete satisfaction.
Zhishang Biography intends to help consumers expand their service by providing the very best solutions and chemical resources.
FAQ
DO YOU ACCEPT SAMPLE ORDER?
We will make samples before mass production. After the samples are approved by the department and users, we will start mass production. During the production process, we will carry out 100% inspection, and then carry out random inspection before packaging.
WHAT’S YOUR MOQ?
Our MOQ is 1kg. But usually we can also accept a smaller quantity, such as 100g, if you need it, provided that the sample fee is 100% paid.
IS THERE A DISCOUNT?
Different quantity has different discount.
HOW TO CONFIRM WHETHER THE PRODUCT QUALITY MEETS OUR NEEDS BEFORE PLACING AN ORDER?
You can consult us to get free samples of some products, you only need to pay the freight or arrange express delivery to us and get the samples. You can send us the product specifications and requirements you need, and we will manufacture products according to your requirements.
DO YOU SUPPLY PRODUCT REPORT?
Yes. We’ll give you product analysis report before shipping.
LEARN MORE ABOUT US
Shandong Look Chemical
Advice on Shipping
1. For products ≤50kg, we recommend using express delivery, which is usually called DDU service (discounted, convenient).
2. For products ≤500kg, we generally recommend air freight, which is usually called FOB, CFR or CIF service (fast and efficient).
3. For products >500kg, we generally recommend shipping by sea, which is usually called FOB, CFR or CIF service (economical, safe).
4. For high-value products, please choose air or express to ensure the safety of product transportation.
Company Info
Zhishang Bio company in China that exports chemicals, Cosmetic raw materials, and Intermediates for over 70 countries now.
And a range of 50,000 products with the best quality and competitive price to meet your different purchasing needs.
The company integrates research and development, production, and trade, with a modern production base, advanced testing equipment, and an experienced technical team to ensure that products reach industry-leading levels in purity, stability, and environmental friendliness.
