Sulfo Cyclohexane Cyanine7 NHS ester CAS 1603861-95-5
Sulfo Cyclohexane Cyanine7 NHS ester CAS 1603861-95-5
- Surfactant Appearance: powder
- Proportion: Minimum 99.0%
- Inventory: In stock
- Sample: In stock
- Zhishang Chemical: Supplier and manufacturer of Sulfo Cyclohexane Cyanine7 NHS ester CAS 1603861-95-5
Basic Info of Sulfo Cyclohexane Cyanine7 NHS ester
| Sulfo Cyclohexane Cyanine7 NHS ester Basic information |
| Product Name: | Sulfo Cyclohexane Cyanine7 NHS ester |
| Synonyms: | Sulfo Cyclohexane Cyanine7 NHS ester;diSulfo-Cy7NHSEster |
| CAS: | 1603861-95-5 |
| MF: | C41H48KN3O10S2 |
| MW: | 846.06 |
What is Sulfo Cyclohexane Cyanine7 NHS ester？
Sulfo Cyclohexane Cyanine7 NHS ester (Chinese name: Sulfonic acid group – Cy7 – succinimide ester, CAS number: 1603861-95-5) is a functionalized near-infrared fluorescent dye, combining water-soluble sulfonic acid groups, highly fluorescent Cy7 chromophore, and NHS responsive ester that responds with amino teams.
The molecular formula of Sulfo Cyclohexane Cyanine7 NHS ester is C ₄₁ H ₄₈ KN ₃ O ₁₀ S ₂, with a molecular weight of 846.06. Its appearance is a dark green powder. Compared with the standard Cy7 dye, this product has significant enhancements – the quantum yield is raised by roughly 20%, and its light stability is stronger. The introduction of the sulfonic acid team offers it superb water solubility, which can be dissolved in numerous polar solvents such as water, DMSO, and DMF, and is especially appropriate for liquid biological experiments.
The core application of Sulfo Cyclohexane Cyanine7 NHS ester lies in the near-infrared fluorescence labeling of biomolecules. Its NHS energetic ester group can respond with free main amines in healthy proteins, antibodies, and peptides under neutral to weakly alkaline conditions (pH 7-9), developing steady amide bonds, thus achieving covalent labeling.
Near-infrared fluorescence (λ_ex ≈ 750 nm, λ_em ≈ 773 nm) has special benefits – the spontaneous fluorescence of biological tissues in this wavelength range is incredibly reduced and the cells penetration deepness is big. For that reason, it is especially ideal for deep tissue discovery experiments such as in vivo imaging and in vivo mapping of tiny animals. At the same time, Sulfo Cyclohexane Cyanine7 NHS ester has high water solubility and low non-specific adsorption, making it an excellent choice for labeling healthy proteins conscious solvents or proteins with variability.
The Sulfo Cyclohexane Cyanine7 NHS ester is sensitive to moisture and light. It needs to be kept in a completely dry, dark, and closed setting at -20 ° C. Under proper storage conditions, its stability can be preserved for a minimum of one year; it can be delivered at space temperature for as much as 3 weeks, yet must be safeguarded from prolonged direct exposure to light.
Product Packaging
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Superior Sulfo Cyclohexane Cyanine7 NHS ester Series | Reliable Quality & Sufficient Supply
Our supply benefits and capabilities
As an expert vendor of Sulfo Cyclohexane Cyanine7 NHS ester, we are cognizant of the core demands of research users for this type of product.
HPLC-MS tests have verified the high purity of our products, which is usually over 95%. Each batch is issued a total top quality inspection (COA) report to make sure consistency between sets in terms of vital indications like pureness, absorption/emission waves, molar coefficients (e.g. 2.4 x105 mol-1 * cm-1), quantum yield (around 0.24) and molar coefficients (e.g. 2.4 x105 mol-1 * cm-1).
Sulfo Cyclohexane Cyanine7 NHS ester is available in a selection of product packaging dimensions, consisting of 1mg and 5mg. It likewise can be found in 10mg and 25mg. This permits a wide variety of does, from basic research study approximately medicine screening. Minimum order quantities are 1mg for research consumers. We likewise provide price cuts on mass acquisitions. Ahead of time, large orders can be used to secure manufacturing capacity and ensure a secure supply.
Expert technical support and accompanying service: We offer in-depth operation manuals that consist of guidelines for the prep work of the labeling barrier and recommendations to maximize labeling efficiency.
Our technological assistance team is readily available to give individually aid with any type of technological issues, such as low labeling efficiency or healthy protein precipitation.
FAQ
The main difference between the two is their water solubility.
Suggested steps: Dissolve Sulfo-Cy7 NHS ester in anhydrous DMSO or DMF to prepare a 10 mM storage solution. Dissolve the target protein in 0.1 M sodium bicarbonate buffer at pH 8.3-8.5 (avoid using amine containing buffers such as Tris and glycine). Mix in a dye to protein molar ratio of 2:1 to 10:1, and react at room temperature in the dark for 1 hour. Common reasons for low labeling efficiency include the presence of free amines in the buffer, low pH value, insufficient dye dosage, or short reaction time. After the reaction, it is necessary to use a desalination column or dialysis to remove free dyes.
It is recommended to use a desalination column (such as Sephadex G-25) for rapid purification, or to use dialysis (molecular weight cut-off selected based on protein size, usually 10-30 kDa) overnight to remove free dyes. After purification, the protein concentration (A280) and dye concentration (A750) can be determined, and the labeling degree (dye/protein molar ratio) can be calculated.
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Sulfo Cyclohexane Cyanine7 NHS ester Supplier and Manufacturer
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